By John A. Lindbo (auth.), John M. Watson, Ming-Bo Wang (eds.)
Studies regarding pathogen-mediated virus resistance in vegetation have been instrumental in supplying a number of the historic observations which finally ended in the very important discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has for the reason that revolutionized examine on plant-virus interactions. In Antiviral Resistance in vegetation: tools and Protocols, professional researchers within the box element a number of the tools that are now common to review the phenomenon of RNA silencing with regards to viral infections of vegetation. those comprise equipment and strategies for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs akin to small interfering RNAs (siRNAs) and microRNAs (miRNAs) in addition to the research and manipulation of virus-induced gene silencing (VIGS) in either monocotyledonous and dicotyledenous crops and using hairpin RNA (hpRNA) transgenes. Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters comprise introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effectively reproducible laboratory protocols, and key pointers on troubleshooting and keeping off identified pitfalls.
Authoritative and useful, Antiviral Resistance in vegetation: tools and Protocols seeks to help scientists within the extra research of this crucially vital botanical trait.
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